Wound healing represents an interactive process which requires highly organized activity

Wound healing represents an interactive process which requires highly organized activity of various cells synthesizing cytokines growth factors and collagen. types I and III were estimated by the surface CDH5 plasmon Aconine resonance method with a subsequent collagenous quantification using electrophoretic and densitometric analyses. Propolis burn treatment led to enhanced collagens and its components expression especially during the initial stage of the study. Less expressed changes were observed after metallic sulfadiazine (AgSD) software. AgSD and having Aconine a smaller intensity propolis stimulated build up of collagenous degradation products. The assessed propolis therapeutic effectiveness throughout quantitatively and qualitatively analyses of collagen types I and III manifestation and degradation in wounds matrix may indicate that apitherapeutic agent can generate beneficial biochemical environment assisting reepithelization. 1 Intro Propolis a naturally occurring resinous compound represents a popular remedy well known for its broad spectrum of biological activities including antimicrobial antioxidant anaesthetic anti-inflammatory and immune-modulatory actions [1-5]. The apitherapeutic agent which is definitely easily available and well tolerated with rare occurrences of allergy and no toxicity is referred to as an excellent candidate for burn management enhancing Aconine fibroblasts proliferation activation and growth capacity [1 6 Today sterling silver sulfadiazine (AgSD) used as an agent of choice in the treatment of burn wound due to broad spectrum of antimicrobial activity can also be responsible for particular considerable adverse reactions [9]. AgSD may not only contribute to the development of argyria inner organ dysfunction (liver spleen and kidney) due to silver systemic build up or determined by sulphadiazine presence dermatitis erythema multiforme rashes and acute hemolytic anemia but also regrettably could be responsible for cytotoxic effect on fibroblasts and keratinocytes [9 10 Described cytotoxic influence may efficiently retard wound healing process fundamental response on cells injury (comprised of four exactly integrated phases such as hemostasis swelling proliferation and redesigning)-requiring highly coordinated activity of various cells [10-14]. Fibroblasts and keratinocytes seem to play pivotal functions during wound healing since their relationships participate in changing Aconine the wound environment from an inflammatory to a synthesis-driven granulation cells [15-17]. Moreover while migrating from your wound margin and proliferating keratinocytes which are involved in reepithelization [6] fibroblasts differentiated into myofibroblasts and involved in wound contraction and extracellular matrix (ECM) redesigning are responsible for the production of ECM parts including proteoglycans glycosaminoglycan elastin and collagen [10]. Collagen like a structurally and functionally pivotal molecule which builds a scaffold in the connective cells is also involved in hemostasis inflammatory response cell growth differentiation and migration [11 18 19 Furthermore collagen participates in cell signaling angiogenesis manifestation of inflammatory cytokines and growth factors and relationships between matrix metalloproteinases (MMPs) and their cells inhibitors becoming the inherent part of reepithelization [11 18 19 Taking into account that collagen types I and III are the main collagen types of healthy skin being mainly expressed during restoration process [19] Aconine the aim of this study was to compare the propolis and metallic sulfadiazine therapeutic effectiveness (in the treatment of thermal accidental injuries) throughout the quantitative and qualitative assessment of the pointed out collagen types build up in the matrix of burnt cells. 2 Material and Methods 2.1 Reagents and Materials The following antibodies were used: polyclonal rabbit anti-human collagen type III antibodies (Rockland Gilbertsville PA USA; no. 600-401-105-0.1) goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Sigma-Aldrich Germany; no. A5420) and mouse monoclonal antibody raised against full size native soluble acid digested pepsin collagen type I of human being source (Santa Cruz Biotechnology Inc. CA USA; no. sc-59773). The following reagents were used: sodium metaperiodate and hydrazide LC-biotin from Thermo Scientific USA; DMSO (dimethylsulfoxide) sodium dodecyl sulfate Triton X-100 Coomassie amazing blue R250 pepsin glycine Immobilon P membranes dithiothreitol Tween 20 (polyoxyethylenesorbitan monolaurate).