We statement the 4. made by Magiorakos et al. (9). DNA was isolated using the Ultra-Clean Microbial DNA isolation kit (MoBio Laboratories Carlsbad CA USA) following the manufacturer’s instructions. Genome sequencing was Zarnestra performed using the PacBio platform at the Genome Quebec facility (Montreal QC Canada) using three SMRT cells. Assembly MAPK10 was carried out using the PacBio SMRT analysis pipeline version 2.2.0 with 93.3× coverage to give a single contiguous genome sequence. The sequence was annotated by the National Center for Biotechnology Information (NCBI) Prokaryotic Genomes Annotation Pipeline. The genome consists of 4 335 793 bases with a G+C content of 39%. There are a total of 4 258 putative genes which include 4 132 protein- 18 rRNA- and 73 tRNA-coding sequences. Nucleotide sequence accession number. The genome sequence of AB030 was deposited in NCBI GenBank under the accession number “type”:”entrez-nucleotide” attrs :”text”:”CP009257″ term_id :”675327552″ term_text :”CP009257″CP009257. ACKNOWLEDGMENTS This work is supported by funding from your Discovery Grants program of the Natural Science and Engineering Council of Canada (A.K. and P.C.L.) the Canada Research Chair program (P.C.L.) and the University or college of Manitoba Research Grant Program (A.K.). Y.A. is usually funded by a graduate scholarship from your Royal Government of Saudi Arabia. Footnotes Citation Loewen PC Alsaadi Y Fernando D Kumar A. 2014. Genome sequence of an extremely drug-resistant clinical isolate of strain AB030. Zarnestra Genome Announc. 2(5):e01035-14. doi:10.1128/genomeA.01035-14. Recommendations 1 Abbo A Navon-Venezia S Hammer-Muntz O Krichali T Siegman-Igra Y Carmeli Y.. 2005. Multidrug-resistant infections in Thailand. Am. J. Infect. Control 37 10.1016 [PubMed] [Cross Ref] 4 Bou G Cerveró G Domínguez MA Quereda C Martínez-Beltrán J.. 2000. Characterization of a nosocomial outbreak caused by a multiresistant strain with a carbapenem-hydrolyzing enzyme: high-level carbapenem Zarnestra resistance in is not due solely to the presence of β-lactamases. J. Clin. Microbiol. 38 [PMC free article] [PubMed] 5 CDC . 2004. infections among patients at military medical facilities treating hurt U.S. support users 2002 MMWR Morb. Zarnestra Mortal. Wkly. Rep. 53 [PubMed] 6 Cristina ML Spagnolo AM Ottria G Sartini M Orlando P Perdelli F Galliera Hospital Group . 2011. Spread of multidrug carbapenem-resistant in different wards of an Italian Zarnestra Hospital. Am. J. Infect. Control 39 10.1016 [PubMed] [Cross Ref] 7 Huys G Cnockaert M Vaneechoutte M Woodford N Nemec A Dijkshoorn L Swings J.. 2005. Distribution of tetracycline resistance genes in Zarnestra genotypically related and unrelated multiresistant strains from different European hospitals. Res. Microbiol. 156 10.1016 [PubMed] [Cross Ref] 8 Fernando D Zhanel G Kumar A.. 2013. Antibiotic resistance and expression of resistance-nodulation-division pump- and outer membrane porin-encoding genes in species isolated from Canadian hospitals. Can. J. Infect. Dis. Med. Microbiol. 24 [PMC free article] [PubMed] 9 Magiorakos AP Srinivasan A Carey RB Carmeli Y Falagas ME Giske CG Harbarth S Hindler JF Kahlmeter G Olsson-Liljequist B Paterson DL Rice LB Stelling J Struelens MJ Vatopoulos A Weber JT Monnet DL.. 2012. Multidrug-resistant extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect. 18 10.1111 [PubMed] [Cross.