Cardioprotection against ischaemia/reperfusion damage in mice may be accomplished by delayed ischaemic postconditioning (IPost) applied while late while 30?min following the starting point of reperfusion. when used over time of myocardial ischaemia enduring 30?min. Delayed IPost used after 30 or 45?min of reperfusion reduced infarct sizes by 36 and 41?% respectively (both shows period of intravenous administration from the inhibitors of RISK [PD-98059 (PD) LY-294002 (LY)] or Safe and sound pathways [AG490 (AG)]. b LY and PD abolished cardioprotection founded by instant … Immunoblotting for success kinases Within an additional group of tests rats underwent 30?min of myocardial ischaemia as well as the hearts were collected in 15?min after sham-IPost or IPost (6 cycles of We/R 10 applied 10?s 10 or 45?min after reperfusion starting point. Rats were randomly assigned to one of the following six groups: BMS 626529 sham-immediate IPost (sham-IPost10″ indicates time of intravenous administration of the mitochondrial KATP channel blocker 5-hydroxydecanoate (5-HD). b 5-HD abolished cardioprotection established by immediate (IPost10″) and early … Statistical analysis Data are reported as mean?±?SD. Data were compared by ANOVA followed by Tukey-Kramer post hoc test or unpaired test as appropriate. Values of P? DP1 20 30 and 45?min of LAD occlusion followed by 120?min BMS 626529 of reperfusion BMS 626529 were 33?±?3 44 and 51?±?8?% respectively (Fig.?1c). Infarcts in animals subjected to 45?min of ischaemia were significantly larger than in animals subjected to 20?min of ischaemia (P?P?P?P?P?P?