Supplementary MaterialsS1 Text message: Supplementary strategies. to a appropriate function (find text message). Rows present different retinas (RXX).(PDF) pcbi.1007691.s006.pdf (333K) GUID:?38F96413-0762-406E-End up being62-B14CFEC421EE S3 Fig: Evaluation of D-V information between retinas. Overlap from the small percentage of cells expressing (still left) M-opsin and (correct) S-opsin aligned towards the changeover midpoint as motivated in the S-opsin appearance profile.(PDF) pcbi.1007691.s007.pdf (320K) GUID:?73D639D9-1ACC-4298-B73F-5F67A11B2673 S4 Fig: S-only cell fraction. Small percentage of cells expressing just S-opsin by placement across the D-V axis. The info in the microscopy evaluation (x) are overlaid with the very best fit (series) to some appropriate function (find text message). Rows present different retinas (RXX).(PDF) pcbi.1007691.s008.pdf (329K) GUID:?A3865C2F-8071-4081-AF00-BE0083C2B988 S5 Fig: Correlation between S- and M-opsin in retinal cells. Joint possibility distributions for the plethora of S-opsin (blue strength) and M-opsin (green strength) in cells. Rows present different retinas (RXX). Shades range between log_10[P] = ?2 (white/yellow) to log_10[P] = ?6 (crimson/dark).(PDF) pcbi.1007691.s009.pdf (382K) GUID:?790B9140-A20D-4D3C-AA62-C1961789A71D S6 Fig: Relationship between S- and M-opsin in retinal cells. Joint possibility distributions for the plethora of S-opsin (blue strength) and M-opsin (green strength) in cells. Columns present cells binned from four different locations according to length from the changeover midpoint. Rows present different retinas (RXX). Shades range between log_10[P] = ?2 (white/yellow) to log_10[P] = ?4 (crimson/dark).(PDF) pcbi.1007691.s010.pdf (318K) GUID:?ECD8FC54-0EA1-4DA1-B199-2BF77150A29C S7 Fig: Appearance of S- and M-opsin in retinal cells. Possibility distribution for the plethora of (still left) M-opsin and (correct) S-opsin in cells by length from the transition midpoint. Rows show different retinas (RXX). Colors Polygalasaponin F range from log_10[P] = 0 (white/yellow) to log_10[P] = ?4 (red/black).(PDF) pcbi.1007691.s011.pdf (318K) GUID:?9DD3A656-C082-4A4C-B871-258FD37F15B8 Polygalasaponin F S8 Fig: Fitting of cell expression intensity data. Mean intensity in all cells of (left) M-opsin and (right) S-opsin by position along the D-V axis. The data from your microscopy analysis (x) are overlaid with the best fit (collection) to a fitted function (observe text). Rows show different retinas (RXX).(PDF) pcbi.1007691.s012.pdf (327K) GUID:?C9B440F1-F4BB-4CDC-BA5B-92506DC9DF2F S9 Fig: Expression in modeled cell populations. Mean portion of cells in various cell populations along the D-V axis from numerical simulations of the model. Plots show the mean value computed from 100 impartial simulations.(PDF) pcbi.1007691.s013.pdf (220K) GUID:?F744C70A-F5C4-452E-BE5A-46B8A27870F0 S10 Fig: Opsin concentrations in modeled cells. Probability distribution of the large quantity of S-opsin (blue intensity) and M-opsin (green intensity) in cells along the D-V axis from numerical simulations of the model. Distributions were computed from 100 impartial simulations.(PDF) pcbi.1007691.s014.pdf (215K) GUID:?0323C564-7560-4166-8322-23A0DA919159 S11 Fig: Correlation between S- and M-opsin in modeled cells. Joint probability distributions for the large quantity of S-opsin (blue intensity) and M-opsin (green intensity) in cells located in 250m wide bins along the D-V axis. Colors range from log_10[P] = ?2 (white/yellow) to log_10[P] = ?5 (red/black). Distributions were computed from 100 impartial simulations. The low density tails leading to 0,0 are from cells that were sampled during the process of switching phenotypes.(PDF) pcbi.1007691.s015.pdf (217K) GUID:?A5B7B638-4BF0-4498-ABB4-80AD8598AB99 S12 Fig: Analysis of pixel intensities in images of THR2 cells. Polygalasaponin F (left) Joint probability distribution of the blue and green intensity of pixels located either inside of cell boundaries (RXX CELL) or the background outside of cells (RXX BG) as indicated. Colors range from log_10[P] = 0 (white/yellow) to log_10[P] = ?8 (red/black). (center) Probability for any pixel of the indicated type to have a particular blue intensity (solid collection) compared with the distribution for all those pixels (dashed collection). (right) The same for Mouse monoclonal antibody to L1CAM. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS(hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia,shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesionmolecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migrationand in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arisingfrom neuroectoderm green intensity. THR2 cells do not exhibit green expression above background.(PDF) pcbi.1007691.s016.pdf (412K) GUID:?ABDC327C-CA7B-4E99-90E7-021A4C3452CB S13 Fig: Expression of S-opsin in THR2 retinal cells. Probability distribution for the large quantity of S-opsin in cells by distance along the D-V axis. Rows show different THR2 retinas (RXX). Colors range from log_10[P] = 0 (white/yellow) to log_10[P] = ?4 (red/black).(PDF) pcbi.1007691.s017.pdf (33K) GUID:?8ADB79B0-CC5B-4A71-93FD-69E58631D743 S14 Fig: Mean retina description. Comparison of the fits for individual retinas (dashed lines) with our hypothetical mean retina used for model parameterization (solid collection) along the D-V axis. The top row shows an evaluation from the small percentage of cells expressing S- and M- opsin, respectively. The center row displays the small percentage of FD(S) cells. Underneath row displays the mean M- and S-opsin appearance strength, respectively.(PDF) pcbi.1007691.s018.pdf (222K) GUID:?BE6B49E2-9572-466A-BB60-6036D0E2F5BF S15 Fig: Ideal fit parameterization. Evaluation of the greatest suit model parameterization (blue) with the hypothetical mean retina (black)..